Research directions: molecular oncology and biotechnology; research of optical parameters of cells and markers; cultivation of normal and tumor cell lines, study of their metabolism, testing of bioactive substances and antitumor drugs; conducting molecular genetic studies, analytical and preparative chromatography, immunoenzymatic, biochemical and immunoturbidimetric analyses; performing microscopic studies (hematology, cytology, histology, immunology, genetics).
Scientific equipment: Optical and cell analyzers: flow cytofluorimeter Beckman Coulter “DxFLEX” model B5-R0-V0 (Beckman Coulter Biotechnology (Suzhou) Co. Ltd., China) (laser with excitation wavelength 488 nm; 5 fluorescent detectors; sensitivity to FITC ≥30 molecules equivalent soluble fluorochrome (MESF), to PE ≥10 MESF); multifunctional reader Synergy HT, model SIAFRTD (Bio-Tek Instruments, Winooski, VT, USA) (measurement of fluorescence, luminescence and absorption in 6–384-well plates). Equipment for PCR and chromatography: real-time PCR system Real-Time PCR Applied Biosystems 7500 (Thermo Fisher Scientific; USA) (5-color detection, 96-well format); liquid chromatograph Agilent 1200 (Agilent Technologies; USA, Germany) with diode-array (190-950 nm) and fluorescent detectors (flow rate up to 10 ml/min). Hematological and biochemical analyzers: automatic analyzer GBG ChemWell 2900 with RCA module for reagent temperature stabilization (Awareness Technology, Inc.; USA) (over 220 tests/hour, spectral measurement range 320-900 nm); automatic hematological analyzer BC-2800VET (Mindray, China) (18 parameters for 13 animal species); automatic hematological analyzer PCE-210 (ERMA Inc., Japan) (18 parameters); blood gas analyzer ABL 800 FLEX (Radiometer, Denmark). Microscopic equipment: microscope AXIO SCOPE.A1 (Carl Zeiss Microscopy GmbH, Germany) (bright/dark field, phase/polarization contrast, fluorescence, camera with 5 MP resolution); fluorescent (luminescent) microscope MS 300 FX (Infinitive; Austria) (magnification up to 1600x, spectral range 420-650 nm); optical microscope Zeiss Axio Imager 2 for biological research (Carl Zeiss AG, Germany) (camera Axiocam 712 color, ZEN Pro software, research methods: bright/dark field, phase, polarization and differential interference contrast). Spectrophotometers: diode-array Agilent 8453 (Agilent Technologies; USA) (190-1100 nm, resolution 1.63); single-beam scanning spectrophotometer Helios Gamma (Thermo Electron; UK) (190-1100 nm). Auxiliary and preparative equipment: CO2 incubator CelCulture CCL-170B-8-HHS (Esco Lifesciences Group, Esco Micro Pte. Ltd., Singapore) (170 l, high-temperature sterilization); ultracentrifuge Sorvall WX100 Ultra (Thermo Fisher Scientific, USA) (up to 100,000 rpm, acceleration up to 802,000 g); refrigerated centrifuge MIKRO 22R (HETTICH, Germany); automatic scales Ecplorer Pro EP214C IC (Ohaus, USA); freezer “MYBIO” (from -80 °C to -82 °C) (DAIREI A/S, Denmark); water purification system “Simplicity” (Millipore, Germany).
Services: registration of single-cell parameters (flow cytofluorimetry method); cultivation of normal and tumor cell lines and production of cell mass; multiplex polymerase chain reaction (PCR) (gene expression assessment, mutation screening, SNP typing); analytical and preparative chromatography; determination of tumor markers, hormones, diagnosis of viral/bacterial infections and autoimmune diseases, determination of enzyme activity; measurement of fluorescence, luminescence and absorption (optical density) of materials; quantitative analysis of formed elements of animal and human blood; high-precision optical and fluorescent microscopy; ultracentrifugation; deep freezing of samples.