| Publications on the project |
1111 Nanohyperthermia of malignant tumors. II. In vivo tumor heating with manganese perovskite nanopartickes |
Authors: | L. Bubnovskaya, A. Belous, S. Solopan, A. Podoltsev,
I. Kondratenko, A. Kovelskaya, T. Sergienko, S. Osinsky | |
Summary: | Objectives: To evaluate the ability of manganese perovskite nanoparticles (lanthanum-strontium manganite) to heat the tumor tissue in vivo under action of external alternating magnetic field. Materials and Methods: The magnetic fluid on the basis of nanoparticles of perovskite manganite was tested in the heating experiments using of alternating magnetic field of frequency 300 kHz and amplitude 7.7 kA/m. Guerin carcinoma was transplanted into the muscle of rat. Magnetic fluid was injected intramuscularly or intratumorally. Temperature was measured by copper-constantan thermocouple. Results: Temperature of magnetic fluid was increased by 56 °C for 10 min of alternating magnetic field action. Administration of magnetic fluid into the muscle followed by alternating magnetic field resulted in the elevation of muscle temperature by 8 °C after 30 min post injection. Temperature of the tumor injected with magnetic fluid and treated by alternating magnetic field was increased by 13.6 °C on the 30 min of combined influence. Conclusion: In vivo study with rat tissue has demonstrated that magnetic fluid of manganite perovskite injected in the tumor increases the tumor temperature under an alternating magnetic field. Obtained results emphasize that magnetic fluid of manganite perovskite can be considered as effective inducer of tumor hyperthermia. | |
Keywords: | manganese perovskite, magnetic fluid, alternating magnetic field, tumor, hyperthermia. | |
Edition: | Experimental Oncology | | | 2012,
336 - 339,English |
1111 Synthesis and Properties of Ferromagnetic Nanoparticles for Potential Biomedical Application |
Authors: | А. Belous, S. Solopan, O. Yelenich, S. Osinsky,
L. Bubnovskaya, L. Bovkun | |
Summary: | Nanocrystalline and slightly agglomerated
particles of ferromagnetic compounds with spinel Fe3O4 and
perovskite La1-xSrxMnO3 structure have been synthesized by coprecipitation from nonaqueous solutions. Magnetic properties of the synthesized nanoparticles have been investigated. It was
found that the obtained nanoparticles exhibit superparamagnetic
behavior. Developed magnetic fluids based on synthesized
nanoparticles have shown promise in hyperthermic cancer
therapy, both in vitro and in vivo. | |
Keywords: | spinel, perovskite, nanoparticle, co-precipitation,
hyperthermia. | |
Edition: | 2014 IEEE 34th International Scientific Conference on Electronics and Nanotechnology (ELNANO), Proceedings of Conference | | | 2014,
245-249,English |
1111 Magnetic Fluid Hyperthermia of Rodent Tumors Using Manganese Perovskite Nanoparticles |
Authors: | Larissa Bubnovskaya, Anatolij Belous, Sergej Solopan, Antonina Kovelskaya, Lyudmila Bovkun, Alexander Podoltsev, Igor Kondtratenko, and Sergej Osinsky | |
Summary: | Purpose. To test the antitumor activity of magnetic fluid (MF) on the basis of substituted lanthanum-strontium manganite
nanoparticles combined with alternating magnetic field (AMF) in experiments with transplanted tumors. Materials and Methods.
MF with a size of nanoparticles of 30–40 nm in aqueous agarose solution was investigated.The ability of MF to heat tumor under AMF (300 kHz, 7.7 kA/m) was tested in vivo with rodent tumors (Guerin carcinoma,Walker-256 carcinosarcoma, and Lewis lung carcinoma (3LL)). Results. Single administration of MF into the tumor at a dose of 150mg/kg (rats) or 200mg/kg (mice) followed by AMF within 20–30 min (treatment was repeated 3-4-fold) has resulted in the complete regression of tumor in the 35% of rats and 57% of mice.Administration of MFalone or action ofAMF alone has not resulted in tumor growth inhibition.The chemomodifying effect of nanohyperthermia was determined, in particular for cisplatinum: thermal enhancement ratio was 2.0. It was also observed that nanohyperthermia has resulted in the absence of 3LL metastases in 43% of mice. Conclusions. MF on the basis of lanthanumstrontium manganite may be considered as an effective inductor of tumor local hyperthermia. | |
Keywords: | | |
Edition: | Journal of Nanoparticles | | | 2014,
1-9,English |
1111 Nanoparticles of spinel and perovskite ferromagnets and prospects for their application in medicine |
Authors: | A. G. Belous, S. O. Solopan, O. V. Yelenich, A. I. Tovstolytkin, T. V. Kolodiazhnyi, S. P. Osinsky, and L. N.
Bubnovskaya | |
Summary: | Abstract. In this work, nanoparticles of La0.75Sr0.25MnO3 compounds with perovskite structure and AFe2O4 (A = Mn, Fe,
Co, Ni, Zn) with spinel structure have been synthesized by precipitation from diethylene glycol and microemulsion using
Triton X-100 surfactant. Comparative X-ray diffraction and magnetic studies of the synthesized nanoparticles have been
carried out. Magnetic fluids prepared from synthesized nanopowders have been characterized by calorimetric
measurements of specific loss power (SLP). | |
Keywords: | | |
Edition: | AIP Proceedings | | | 2014,
13-18,English |
1111 Nanoparticles of Ferromagnetic Materials and Possibilities of their Application in the Hyperthermia of Malignant Tumors |
Authors: | A. Belous, S. Solopan, A. Yelenich, L. Bubnovskaya, S. Osinsky | |
Summary: | Slightly agglomerated, crystalline nanoparticles of
ferromagnetic compounds with spinel and perovskite
La1-xSrxMnO3 structure have been synthesized by the sol-gel
method, precipitation from glycol solutions and using
microemulsion technique. “Core/shell” La1-xSrxMnO3/SiO2
stucture have been developed. Magnetic properties of the
synthesized nanoparticles have been investigated. It was shown
that the obtained nanoparticles exhibit superparamagnetic
properties. It has been found that the synthesized nanoparticles
have promise in hyperthermia of cancer cells. | |
Keywords: | spinel, perovskite, nanoparticle, precipitation,
hyperthermia. | |
Edition: | Conference Proceedings of 2013 IEEE XXXIII Int Sci Conf “Electronics and Nanotechnology (ELNANO)”, Kyiv, Ukraine, April 15-18, 2014, pp.245-249. | | | 2013,
245-249,English |
1111 NANOHYPERTHERMIA OF MALIGNANT TUMORS. I. LANTHANUM-STRONTIUM MANGANITE MAGNETIC FLUID AS POTENTIAL INDUCER OF TUMOR HYPERTHERMIA |
Authors: | S. Solopan, А. Belous, A. Yelenich, L. Bubnovskaya,
A. Kovelskaya, A. Podoltsev, I. Kondratenko, S. Osinsky | |
Summary: | Objectives: To synthesize magnetic particles of lanthanum-strontium manganite, prepare the magnetic fluid (MF), evaluate
the generation of heat by particles and determine their common toxiсity. Methods: Nanoparticles based on the solid solutions of lanthanum-strontium manganite (La1-xSrxMnO3) have been synthesized by a sol-gel method. Conventional methods of experimental oncology were used. Results: Nanoparticles of ferromagnetic materials on the basis of solid solutions of lanthanum strontium manganite by sol-gel method were synthesized. It was shown the possibility to regulate the aggregate form of particles that are formed during the synthesis. Magnetic fluid based on the synthesized nanoparticles and water solutions of agarose have been
produced. It was shown the possibility to heat this magnetic fluid up to 42–45 °С in externally applied alternating magnetic field (AMF) operated at 100–400 kHz. It was determined that under long-term influence of AMF nanofluid is heated up to temperature which is not over that of magnetic phase transition. It was detected that magnetic powder as well as fluid have not displayed acute toxicity or side effects (intraperitoneal or intratumoral administration) in animals either intact or with transplanted tumors. Conclusions: Possibility of synthesized magnetic fluid to generate heat in externally applied AMF as well as lack of side effects allow to consider its as a potential mean for tumor hyperthermia (HT). | |
Keywords: | ferromagnetic materials, sol-gel synthesis, hyperthermia, magnetic fluid, nanosized particles. | |
Edition: | Experimental Oncology | | | 2011,
130-135,English |
| The events in the framework of the project |
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1111 4.2. Physical and chemical basis of synthesis and formation properties of nanomaterials medical Purpose:to determine the basic characteristics of nanocomposites on the basis of complicated ferromagnetic oxides under influence of the factors of tumor microenvironment Expected results:Other (to increase production, improve working conditions, environmental improvement, energy savings, material savings, reduced equipment wear, increase productivity, improve efficiency of diagnosis and treatment, etc.) Stage 1:Investigation of peculiaritites of of nanopowders synthesis (precipitation from nonaqueous solutions and sol-gel method) ferromagnetic materials of different types (magnetite (Fe3O4), maghemite (γ Fe2O3), barium hexaferrite BaFe12O19), lanthanum-strontium manganite (La,Sr)MnO3)), and their impact on features of synthetized materials. Detection of presence of alterations of biological effects of nanopowders on cells under "acidic" and hypoxic tumor microenvironment. Stage 2:Investigation of methods for the modification of nanoparticle surface of complicated ferromagnetic oxides with hierarchic architecture and polyfunctional features. Detection of the influence of modification of biocompatibility, dispersity and magnetic features of synthesized materials. Study of nanopowders effects under alternating magnetic field in vivo. Stage 3:Investigation of magnetic biocompatible organic matrixes to create the magnetic fluids on the basis of complicated ferromagnetic oxides and obtained bioorganic matrixes. Detection of the mechanisms of impact of tumor microenvironment on physical and chemical characteristics of magnetic fluids. Stage 4:Investigation of features of nanosized particles of ferromagnetic oxides and magnetic fluids under action of tumor microenvironment factors. Detection of the link between peculiarities of the technology of magnetic nanofluids obtaining and factors of tumor microenvironment and cellular components of stroma. Stage 5:Detection of interrelationship of methods of nanoparticles synthesis and magnetic fluids of ferromagnetic oxides with their features both under air and factors of microenvironment of tumor cells. Optimization of the methods of application of nanopowders and nanofluids taking into account the impact of tumor microenvironment factors on thier biological properties.
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