Publications on the project |
102 Gold nanoparticles genotoxicity at eukaryotic cells by comet-assay method |
Authors: | S.M. Dybkova, L.S. Rieznichenko, T.G. Gruzina, S.I. Shpyleva,
Z.R. Ulberg, V.F. Chekhun
| |
Summary: | Gold nanoparticles (10-45 nm) genotoxicity were investigated at tumor cells (human hystiocytic lymphoma cell line U937) and blood leucocytes by comet assay method. It was shown that 10 nm and 20 nm gold nanoparticles but not 30 nm and 45 nm gold nanoparticles possess genotoxic properties for eukaryotic cells. This data were confirmed by metabolic activation test. The received results allow to define the biosafe sizes of gold nanoparticles for their subsequent use as carriers of antineoplastic preparations in target therapy. | |
Keywords: | | |
Edition: | | | | 2010,
166-170,Ukrainian |
102 Assessment in vivo of the different sizes gold nanoparticles’ genotoxicity |
Authors: | S. M. Dybkova, L.S. Rieznichenko, T.G. Gruzina,
Z.R. Ulberg
| |
Summary: | It is shown that gold nanoparticles with an average with size 20 and 30 nm (in contrast to the nanoparticle with size 10 and 45 nm) do not interact with blood serum albumin and resistant to coagulation in model systems .
By the method of alkaline gel-electrophoresis single eukaryotic cells (“comet-assay”) has been shown that the gold nanoparticles with size 20, 30 and 45 nm have not genotoxic influence on the cells of the liver, kidneys, intestines and bone marrow at a single intravenous injection to laboratory rats. Gold nanoparticles with size 20 nm, in contrast to nanoparticles with size 30 and 45 nm, have genotoxic influences in the spleen.
These data suggest that the gold nanoparticles with size 30 nm is the most biocompatible and biosafety.
| |
Keywords: | gold nanoparticles, blood models system, serum albumin, coagulation, genotoxicity, method of alkaline single cells gel electrophoresis, biocompatibility, biosafety | |
Edition: | Biotechnology | | | 2010,
66-71,Ukrainian |
102 Peculiarities of live cells’ interaction with micro- and nanoparticles |
Authors: | A. Dukhin, Z. Ulberg, V. Karamushka, T. Gruzina | |
Summary: | Experimental evidence collected more than 20 years ago in different laboratories suggests that the
interactions between live biological cells and micro- and nanoparticles depend on their metabolic state.
These experiments were conducted by reputable groups, led by prominent leaders such as H. Pohl of the
USA, who was the inventor of dielectrophoresis, and B. Derjaguin of the Soviet Union who was the leading
author of DLVO theory. The experiments had been mostly conducted with microparticles in the early 1980s.
In the early 1990s, Ukrainian researchers showed that the interaction of live cells with gold nanoparticles
consisted of an initial reversible step that also depended on cell metabolism. They found indirect evidence
that the ion pumps of the cells were responsible for the reversible step. Ion pumps generate a
transmembrane potential, a measurable and widely-used characteristic of the cell's energetic state. The
transmembrane potential, in turn, strongly affects the ζ-potential, as was experimentally discovered 40 years
ago by several independent groups using cell electrophoresis. This relationship should be taken into account
when DLVO theory is considered as the basis for describing the interactions between live cells and micro-
and nanoparticles. Unfortunately, detail theoretical analysis indicates that such modification would not be
sufficient for explaining observed peculiarities mentioned above. That is why distinguished theoreticians
such as Pohl, Frohlich, Derjaguin and others have suggested three theoretical models, presumably to explain
these experiments. These theoretical models should be considered to be complementary to the well-
established concepts developed on this subject in the molecular biology of cells and cell adhesion. This paper
is not a revision of the existing models. It is an overview of the old and forgotten experimental data and
discussion of the suggested theoretical models.
The unusual interaction mechanisms are only specific for live biological cells and serve a dual role: either as a
first barrier to protect the cell from potentially damaging, dispersed particulates, or as a means of
accumulating useful substances. Both functions are critical for the modern problem of nanotoxicology.
| |
Keywords: | | |
Edition: | Advances in Colloid and Interface Science | | | 2010,
60-71,English |
102 102 Gold nanoparticles synthesis and biological activity estimation in vitro and in vivo |
Authors: | L.S. Rieznichenko, S.M. Dybkova, T.G. Gruzina, Z.R. Ulberg, I.N. Todor, N.Yu. Lukyanova, S.I. Shpyleva, V.F. Chekhun | |
Summary: | The aim of present study was the synthesis of gold nanoparticles with different sizes and estimation their biological activity in vitro and in vivo. Materials and methods: Water dispersions of gold nanoparticles with different sizes have been synthesized by Davis’ method and characterized by laser-correlation spectroscopy and transmission electron microscopy methods. The gold nanoparticles interaction with tumor cells has been visualized by confocal microscopy method. The enzymes activity was determined by standard biochemical methods, gold nanoparticles distribution - by atomic-absorption spectrometry, genotoxic influence - by “Comet-assay” method. Results: The gold nanoparticles’ size-dependent accumulation in U937 tumor cells and their ability to modulate U937 cells’ membrane Na+,K+-АТР-ase activity has been revealed in vitro. Gold nanoparticles high affinity to the tumor cells has been determined in vivo on the model of Guerin carcinoma. Conclusions: Obtained results both in vitro and in vivo indicate the perspectives of the synthesized gold nanoparticles water dispersions using in the development of preparations for diagnostic and treatment of tumors. The size-dependent biosafety level of nanoparticles influence on healthy organs has to be taken into account. | |
Keywords: | gold nanoparticles; U937 cells; Guerin carcinoma; affinity; biological activity | |
Edition: | Exp. Oncol. | | | 2012,
25–28,English |
102 Gold nanoparticles as potential agent for target therapy of cardio-vascular system’ pathologies |
Authors: | L.S. Rieznichenko, S.I. Mohnatiy, Т.G. Gruzina, А.М. Vorobyeva, Z.R. Ulberg | |
Summary: | The availability of 30 nm gold nanoparticles usage as agents for target therapy of cardio-vascular pathologies has been shown in vitro and in vivo. | |
Keywords: | gold nanoparticles, myofibrilla, myocardium, modulation, Ca,Mg-АТР-ase activity, target therapy | |
Edition: | | | | 2012,
106-112,Ukrainian |
102 Estimation of autoimmune and genotoxic influence of gold nanoparticles with different sizes |
Authors: | Bobyk V.I., Kapustyan L.M., Morozova L.M., Pogribny P.V., Dybkova S.M., Rieznichenko L.S., Gruzina T.G., Ulberg Z.R., Kovalenko V.P.,
Sydoryk L.L.
| |
Summary: | Water dispersions of gold nanoparticles with average sizes 7, 17 and 57 nm have been synthesized by the method of chemical condensation. The character of immune response of model animals’ organisms on the intraperitoneal injection of the gold nanoparticles has been established via the determination of autoantibodies titre against defensin (mBD-2), chaperonin Hsp60 and heart myosin (Myo). The genotoxicity of gold nanoparticles with average sizes 7 and 17 nm as well as absence of the DNA-damaged action under the influence of 57 nm nanoparticles have been revealed in vitro and in vivo.
On the basis of studied parameters of autoimmune and genotoxic influence on organisms of the model animals 57 nm gold nanoparticles are most perspective for using in technologies of cardio-vascular pathologies’ diagnostic and treatment preparations creation.
| |
Keywords: | gold nanoparticles, autoantibodies, defensin, chaperonin Hsp60, myosin, immune response, genotoxicity. | |
Edition: | Biotechnology | | | 2012,
115-121,Ukrainian |
102 Silver nanoparticles study by criteria of genotoxicity, mutagenicity and influence on probiont bacteria strains of human and animal gastrointestinal tract |
Authors: | T.G. Gruzina, S.M. Dybkova, A.O. Priskoka, L.S. Rieznichenko, Z.R. Ulberg, I.S. Chekman | |
Summary: | With using of high prognostic tests for potential risk assessment of silver nanoparticles application in pharmacology it has been shown safety by criteria of genotoxicity, mutagenicity and influence on probiont strains of human and animal gastrointestinal tract of 20, 30 and 55 nm silver nanoparticles synthesized by the method of chemical condensation in water medium. | |
Keywords: | nanopharmacology, silver nanoparticles, genotoxicity, mutagenicity, probiont strains. | |
Edition: | | | | 2012,
40-46,Ukrainian |
102 Doxorubicin dose for congestive heart failure modeling and the use of general ultrasound equipment for evaluation in rats. Longitudinal in vivo stud |
Authors: | Spivak M., Bubnov R., Yemets I., Lazarenko L., Timoshok N., Vorobieva A., Mohnatyy S., Ulberg Z., Reznichenko L., Gruzina T., Zhovnir V., Zholobak N. | |
Summary: | | |
Keywords: | | |
Edition: | Med. Ultrason | | | 2013,
23-28,English |
102 Nanomaterials risk assessment’ problem and methods of its decision |
Authors: | Yu.I. Kundiyev, Z.R. Ulberg, I.M.Trachtenberg, I.S.Chekman, T.G. Gruzina, S.N. Dybkova, L.S. Rieznichenko, M.L. Marchenko
| |
Summary: | The paper summarizes scientific results, which constitute the basis of methodological guidelines for nanomaterials application’ potential risks assessment as well as development of unified system of their biosafety and biocompatibility analytical-prediction assessment. System biomarkers – characteristics of living organisms which are most sensitive to the toxicological action - are basis of system for nanomaterials biosafety’ testing. Approved system of testing methods can be the basis of normative-methodological base for biosafety assessment of nanomaterials’ application in different directions of human activity.
| |
Keywords: | | |
Edition: | | | | 2013,
177-184,Ukrainian |
102 Use gold nanoparticles for targetted delivery cardiotropic drugs |
Authors: | Mokhnatiy S.I., Vorobyeva G.M., Stupina A.S, Rieznichenko L.S., Gruzina T.G., Zhovnir V.A., Yemets I.M. | |
Summary: | In the experiment in modeling of heart failure in rats shows promising use of gold nanoparticles as agents for targeted delivery of drugs cardiotropic. | |
Keywords: | gold nanoparticles, the model of heart failure | |
Edition: | | | | 2013,
314-317,Ukrainian |
The events in the framework of the project |
102 102 102 102 102 102 102 102 102 102 102 102
|
102 4.1. Biocompatible nanostructured materials: interaction with biological systems, safety use, means and methods controlled delivery Purpose:Development and adaptation of assessment methods for estimation of biocompatibility and biosafety of metal nanoparticles in vitro and in vivo as remedies of diagnostic and target delivery of cardiotropic and antiapoptosis drugs Expected results:Issue of new types of products: methods, theories Stage 1:Estimation in vitro of biocompatibility and biosafety of metal nanoparticles with different sizes Stage 2:Estimation in vivo of biosafety and immunogenicity of gold nanoparticles, which are perspective for cardiotherapy Stage 3:Development of constructions based on gold nanoparticles for target delivery of cardiotropic and antiapoptosis drugs and study of their biological efficiency
Stage 4:Study in vivo in models of heart failure, hypertensia and ischemia of therapeutic efficacy of developed nanoconstructions based on gold nanoparticles
Stage 5:Development of technologies for obtaining of pharmaceutical forms of the nanoconstructions and optimization their therapeutic doses in models of heart failure, hypertensia and ischemia during in vivo studies
|