Publications on the project |
103 Effect of Gold Nanoparticles on Proliferative Properties of SPEV Cells |
Authors: | Pavlovich E.V., Volkova N.A. | |
Summary: | It is important to focus on the problem of the studying of mechanisms of nanoparticles interaction with different cell types depending on their size, shape and surface chemistry. The aim of our research was to study the effect of gold nanoparticles on morphological and functional characteristics of the cells.
Effect of gold nanoparticles (NPs) on the viability, adhesion capacity and apoptosis of embryonic porcine kidney epithelial inoculated line (SPEV) cells was studied. We examined the effect of gold NPs on cell proliferation rate. There was shown a stimulating effect of gold NPs under concentration of 6 μg/ml on SPEV cells when culturing for 3 hours, the number of adhered cells increased in 1.3 times if compared with the control samples. Adhesion after culturing for an hour in the samples with gold NPs at concentration of 12 μg/ml was slowed down. Cell morphology when culturing with gold NPs within the investigated range of concentrations did not differ from the control at all stages of observation. It was shown that stimulating effect manifested at low concentrations of gold NPs during 3 and 4 days of culturing. Thus, the number of cells in samples cultured under concentrations of gold NPs of 3 and 6 μg/ml increased if compared with the control samples in 1.15 and 1.24 (the 3rd day) and 1.15 and 1.23 times (the 4th day), respectively.
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Keywords: | cells, culture, porcine embryonic kidney epithelial inoculated line, gold nanoparticles, polysaccharides, apoptosis, necrosis, cytofluorimetry, culturing, adhesion, proliferation, in vitro, light microscopy, 7AAD, Annexin, stimulation, inhibition, suspension. | |
Edition: | Nanocomposites, Nanophotonics, Nanobiotechnology, and Applications. - Springer Proceedings in Physics | | | 2014,
,English |
103 Europium oxide nanoparticles as fluorescent labels cell cultures |
Authors: | Pavlovich E.V. | |
Summary: | | |
Keywords: | | |
Edition: | Biotechnologia Acta | | | 2014,
117-122,Russian |
103 Cryopreservation of human fibroblasts in the presence of gold nanoparticles |
Authors: | Pavlovich E.V, Volkova N.A., Goltsev A.N. | |
Summary: | | |
Keywords: | | |
Edition: | | | | 2014,
203-207,Russian |
103 The processes of adhesion and proliferation of cell line SPEV for the influence of europium oxide nanoparticles |
Authors: | Pavlovich E.V., Volkova N.A., Stepanyuk L.V., Goncharyuk E.I. | |
Summary: | | |
Keywords: | | |
Edition: | Biotechnologia Acta | | | 2013,
119-124,Ukrainian |
The events in the framework of the project |
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103 4.1. Biocompatible nanostructured materials: interaction with biological systems, safety use, means and methods controlled delivery Purpose:Research of interaction features and biocompatibility of nanosize particles with the cultivated cells Expected results:Release of new product: technology Stage 1:Study of the morpho-functional state, changes in proliferative potential of SPEV cell culture in the presence of metal nanoparticles, investigation of the possible toxic effects of nanoparticles. Stage 2:1. Study of the effect of nanoparticles on characteristics of cells in culturing.
2. Research of term preservation of nanoparticles on SPEV cell culture after long-term culturing.
3. Effect of nanoparticles on the morpho-functional state and proliferative potential of SPEV cell culture.
4. Оцінка впливу інкорпорованих наночасток на апоптотичні процеси в клітинах. Stage 3:1. Study of the effect of gold nanoparticles on morphological and functional characteristics of cells.
2. Research of conformational changes of proteins and nucleic acids after nanoparticles interaction.
3. Effect of gold nanoparticles on the cell apoptosis. Stage 4:Research of gold nanoparticles interaction on proliferative potential of cells. Stage 5:The effect of NPs Au with cryopreserved fibroblasts on regenerative processes in animals was researched.
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