Publications on the project |
062 The heterocoagulation of platinum nanoparticles modified polysaccharides and functionalized folate with cancer cells |
Authors: | VR Estrela-Llopis, AV Chevichalova, EM Fesenko, GI Dovbeshko, PM Litvin. | |
Summary: | The method for synthesis of targeted platinum nanoparticles (PtPS–F) func-tionalized by folate and modified by polysaccharides is developed, and the effect of these polysaccharides on tumour cells of different carcinogenesis is studied. Optimal conditions for the PtPS–F synthesis are revealed, based on spectral and electrosurface studies of formation of the adsorption layer of polysaccharides and folate on the surface of nanoparticles. The optimum con-centration of reactants and the conditions (time, temperature, sequence of mixing) for the synthesis of the PtPS–F nanocomposites are established. The toxic effect of nanocomposites on the cancer cells and its selectivity in com-parison with healthy cells are determined by means of investigation of heter-ocoagulation and kinetics of cytotoxic effect. The methods of atomic force, electron and optical microscopies as well as IR-spectroscopy are used. | |
Keywords: | platinum nanoparticles, polysaccharides, folate, blood cancer cells, ovarian cancer cells lines A2780, Ehrlich carcinoma, heterocoagulation, cytotoxic effect | |
Edition: | Nanosystems, Nanomaterials, Nanotechnologies | | | 2013,
741–755,Russian |
062 Platinum Nanoparticles with Adsorptive Layer of Chlorella vulgaris Polysaccharides Inactivate Tumor Cells of Ascitic Ehrlich Carcinoma, Ovarian Cancer, and Leukemia |
Authors: | A.V. Сhevichalova, V.R. Estrela-Llopis, N.A. Trigubova, E.D. Shishko, P.M. Litvin | |
Summary: | In comparison (with) nanogold it is known not so much works on the application of nanoplatinum in cancer therapy. The purpose of this work - to investigate the aggregation of platinum nanoparticles with different cancer cells and its effects on cells vital activity.
The “green” synthesis of platinum nanoparticles from hexachloroplatinic acid solution was conducted, using as the reducing agent ascorbic acid for platinum nanoparticles, and as the reducing-stabilizer – polysaccharides of Chlorella for the same nanoparticles modified by polysaccharides adsorption layer. Here we take into consideration the known properties of Chlorella’s polysaccharides as immunostimulators, inhibitors of proliferation and activators of apoptosis of cancer cells.
It was studied the interaction of NP PtPS with cancer cells (ascitic Ehrlich carcinoma, ovarian cancer A2780 human line, leukemia - transformed β-lymphocytes-cells Namalwa line, and for comparison - with lymphocytes of healthy human) on the basis of a complex of methods, including studies of heterocoagulation of cells and particles and kinetics of cell death (cytotoxic effect).
This study showed that for the first 20 minutes of contact were died over 50% cells of ovarian cancer and Ehrlich carcinoma. For leukemic cells such time is 2 hours. It was established the selectivity of NP PtPS action - more than 90% of healthy lymphocytes maintain their viability after 2 hours of contact. Bioconjugates of platinum nanoparticles with polysaccharides can be the basis of the perspective drug for cancer therapy. | |
Keywords: | nanoplatinum, ovarian cancer A2780 human line, ascitic Ehrlich carcinoma, leukemia - transformed β-lymphocytes-cells Namalwa line, heterocoagulation, polysaccharides, cytotoxicity. | |
Edition: | Nanocomposites, Nanophotonics, Nanobiotechnology, and Applications. Series: Springer Proceedings in Physics | | | 2014,
293-309,English |
062 Heterocoagulation of Polysaccharide Coated Platinum Nanoparticles with Ovarian Cancer Cells |
Authors: | V.R. Estrela_Llopis A.V. Chevichalova, N.A. Trigubova, E.V. Ryzhuk. | |
Summary: | Platinum nanoparticles coated with adsorption layers of polysaccharides of Chlorella vulgaris (PtPS) have been synthesized and their heterocoagulation with human ovarian cancer cells of the A2780 line has been studied. It has been shown that the characteristic time of the aggregation of the cells with PtPS nanoparticles is 20–25 min, which corresponds to fast barrierless coagulation. The cytotoxic effect of PtPS nanoparticles reaches saturation and more than 50% of the cells die within this time period. Cytomorphological
investigations have shown that A2780 cells with doubled resistance to an antitumoral antibiotic, doxorubicin,
are more sensitive to the toxic action of PtPS nanoparticles than nonresistant cells are. An electrokinetic control test has been proposed for cancercell resistance based on the measurement of variations in the ζ-potential of nanoparticles due to adsorption of watersoluble metabolites of the cells on a particle surface. | |
Keywords: | | |
Edition: | Colloid Journal | | | 2014,
1-14,Russian |
062 Extracellular Biomineralization and Synthesis of Gold and Platinum Nano- and Microcrystals in Polysaccharide Aqueous Solutions |
Authors: | V.R.Estrela-Llopis, T.I.Borodinova, I.N.Yurkova. | |
Summary: | | |
Keywords: | | |
Edition: | Nano-Science: Colloidal and Interfacial Aspects | | | 2010,
307-368,English |
062 The heterocoagulation of bioconjugates and nanocomposites based on platinum nanoparticles with normal and transformed cells of different carcinogenesis |
Authors: | A.V. Chevichalova, V.R. Estrela-Llopis, N.A. Tregubova, P.M. Litvin. | |
Summary: | | |
Keywords: | | |
Edition: | Reports of the IV International Conference on Colloid Chemistry and Physicochemical Mechanics (IC-CCPCM 2013) | | | 2013,
,English |
062 Influence of the substrate on the growth of ultrathin gold films |
Authors: | AA Marchenko, VR Estrela-Llopis, AG Naumovets | |
Summary: | | |
Keywords: | | |
Edition: | nanostructural materials | | | 2010,
,Russian |
062 Bioconjugates and nanocomposites based on nanoparticles of gold and platinum - the basis of new formulations for cancer therapy. |
Authors: | VR Estrela-Llopis, TI Borodinova, NA Tregubova, AV Chevichalova, AI Olejnik. | |
Summary: | | |
Keywords: | | |
Edition: | nanostructural materials | | | 2010,
,Russian |
The events in the framework of the project |
062 062 062 062 062 062 062 062 062
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062 2.4. Colloidal nanoscale systems Purpose:To investigate the effect bioconjugates (BC) based on gold and silver nanoparticles on cancer cells and tumors (due to heterocoagulation BC with tumor), to establish the cytotoxic effect of the BC and their optimal concentrations and doses to further create on their basis anticancer drugs Expected results:Issue of new types of products: methods, theories Stage 1:To investigate heterocoagulation, kinetics of heterocoagulation, the kinetics of cytotoxic effect bioconjugates - of gold nanoparticles with adsorption layer of polysaccharides and nanocomposites - nanoparticles of gold with adsorption layer of doxorubicin and an outer layer of polysaccharides in vitro experiments with cancer cells. Stage 2:To investigate heterocoagulation, kinetics of heterocoagulation, the kinetics of cytotoxic effect bioconjugates - of silver nanoparticles with adsorption layer of polysaccharides and nanocomposites - nanoparticles of silver with adsorption layer of doxorubicin and an outer layer of polysaccharides in vitro experiments with cancer cells. Stage 3:To elaborate synthesis method of targeted gold nanoparticles and nanocrystals functionalized folate and modified polysaccharides and explore their impact on the tumor cells of various oncogenesis. Stage 4:To elaborate synthesis method of targeted platinum nanoparticles and nanocrystals of PtCl2 functionalized folate and modified polysaccharides and explore their impact on the tumor cells of various oncogenesis. Stage 5:To elaborate synthesis method of biomarkers based on nanoparticles and nanocrystals of gold and silver functionalized folate and modified polysaccharide for use in biosensorics and visualization of tumor cells.
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