Publications on the project |
097 Optimization of bioselective membrane of amperometric enzyme sensor on basis of glucose oxidase using NH2-modified multi-wall carbon nanotubes |
Authors: | Biloivan O.A., Rogaleva N. S., Korpan Y. I. | |
Summary: | Aim. To investigate a possibility of application of multi-wall carbone nanotubes modified with NH2-groups(MWCNT-NH2) for creation of sensitive elements of the amperometric biosensor based on immobilized oxidoreductases, in particular, glucose oxidase (GOD). To study electrochemical properties of the membranes obtained. Methods. Experiments were carried out with am- perometric methods using the ìStat 200 device («DropSens», Spain). The enzymes were immobilised in glutaraldehyde vapour. Results. The method of formation of bioselective matrix based on immobilised GOD with MNP-NH2 on the surface of gold amperometric electrodes was optimised. Optimal working conditions of the biosensor developed were determined. Conclusion. MWCNT integration into a bioselective matrix improves the biosensor analyticalcharacteristics which means: higher signal value, wider linear range of glucose analysis, and possibility ofsubstrate determination in wide range of working potential. Keywords: NH2-modified multi-wall carbone nanotubes, amperometric biosensor, oxidoreductase, glucose oxidase | |
Keywords: | NH2-modified multi-wall carbone nanotubes, amperometric biosensor, oxidoreductase, glucose oxidase | |
Edition: | Biopolymers and Cell | | | 2010,
56-61,English |
097 Carbon nanomaterials: a study of the characteristics and application in biosensor technology |
Authors: | Biloivan O.A., Duda T.I., Vasil’ev O.O., Garbuz V.V., Muratov V.B., Shkotova L.V., Zhybak M.T., Boychuk Y.V., Bardakov B.V., Korpan Y.I. | |
Summary: | | |
Keywords: | Biosensor, Carbon nanomaterials, Nanocomposite Enzyme Membranes | |
Edition: | | | | 2014,
Редкол.: А.Г. Наумовец (глав. ред.) ; НАН Украины. – К. : Академпериодика, 2014. – C. 541-545,Russian |
097 Amperometric biosensors, modified with multiwalled carbon nanotubes for glucose determination |
Authors: | Rogaleva N.S., Shkotova L.V., L’vova O.V., Garbuz V.V., Muratov V.B., Duda T.I., Vasil’ev O.O., Korpan Y.I., Biloivan O.A. | |
Summary: | Multiwalled carbon nanotubes were applied to improve the analytical characteristics of amperometric biosensors for glucose determination. Attested samples of aminated and carboxylated multiwall carbon nanotubes were suspended and used for modification of amperometric biosensors based on immobilized glucose oxidase and amperometric transducers С220АТ of Drop Sens production. The bioselective glucose oxidase based membrane was formed on the surface of working electrode by several methods: covalent crosslinks and co-immobilization of horseradish peroxidase in bovine serum albumin gel in vapors of glutaraldehyde, electropolymerization to the conductive polymer film of poly(3,4-ethylenedioxythiophene). Electrochemical measurements were performed by the device μStat 200 («DropSens», Spain). The amperometric biosensors with bioselective membrane based on multiwall carbon nanotubes have been shown to be advantageous as compared to those without carbon nanotubes in terms of higher sensitivity, lower working potential, wider range of detectable analyte concentrations. The optimized biosensor based on membrane with multiwall carbon nanotubes and glucose oxidase was used for determination of glucose in wine. It was shown a reliable correlation between the biosensor method and high-performance liquid chromatography method results. | |
Keywords: | multiwall carbon nanotubes, biosensors, glucose, glucose oxidase, nanocomposite membranes | |
Edition: | Biotechnology | | | 2012,
P. 53–61,Ukrainian |
097 Glucose biosensor based on screen-printed electrodes and glucose oxidase layer modified by MWCNT-NH2 |
Authors: | Rogaleva N., Korpan Y., Biloivan O. | |
Summary: | Glucose biosensor based on screen-printed electrodes and glucose oxidase (GOx ) layer modified by aminated multi-walled carbon nanotubes (MWCNT-NH2) has been developed. The optimal membrane composition for enzyme immobilization by covalent binding in saturated GA vapors was determined as 0.1% of GOx , 0.25% of MWCNT-NH2, 6.0% of bovine serum albumin (BSA), and 5.0% of glycerol. It has been shown that the integration of MWCNT-NH2 into GOx membrane is an effective approach to extend the linear dynamic range of glucose determination and to improve the biosensor sensitivity. However, analytical performances of the MWCNT-NH2 modified biosensor are restricted in terms of lowering its detection limit | |
Keywords: | NH2-Modified Multi-Walled Carbon Nanotubes, Amperometric Biosensor, Glucose Oxidase, D-Glucose | |
Edition: | Sensor Letters | | | 2011,
2356-2359,English |
097 Влияние газообразующих примесей на теплоемкость нанокристаллического алмаза детонационного синтеза |
Authors: | V. B. Muratov, O. Vasil’ev, V. V. Garbuz, Т. І. Duda | |
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Edition: | | | | 2011,
23-31,Russian |
097 097 Thermodynamic properties of multiwalled carbon nanotubes |
Authors: | Muratov, V. B., Vasil’ev, O. O., Kulikov, L. M., Garbuz, V. V., Nesterenko, Yu. V., Duda, T. I. | |
Summary: | | |
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Edition: | Journal of Superhard Materials | | | 2012,
173–178,English |
097 Influence of surface area for the heat capacity of the detonation synthesis nanodiamond |
Authors: | Vasil’ev O. О., Muratov V. B. , Duda Т. І. | |
Summary: | | |
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Edition: | | | | 2012,
104,Russian |
097 3D – Nanostructured scaffold electrodes based on Single-Walled Carbon Nanotubes and Nanodiamonds for high performance biosensors |
Authors: | Singh M., Holzinger M., Biloivan J., S. Cosnier | |
Summary: | We report a novel strategy for controlled nanostructuring of electrodes surfaces using single-walled nanotubes and nanodiamonds in order to form highly porous structures for biosensors applications. These nanostructures were obtained by subsequent deposition of nanotubes and nanodiamonds followed by functionalization of this assembly with pyrenenitrilotriacteic acid (NTA) via dip coating. The electropolymerizaton of the pyrene derivative
leads to a reinforcement of the structure and allows simultaneously the immobilization of biotin labeled bioreceptor units via coordinative affinity interactions. By using biotin modified glucose oxidase (B-GOx) as model bioreceptor, we could obtain impressive maximum current densities and sensitivities up to 465 µA cm_2 and 85.78 mA M_1 cm_2, respectively.
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Edition: | Carbon | | | 2013,
349-356,English |
097 097 Biosensors. A quarter of a century of R&D experience |
Authors: | Soldatkin A. P., Dzyadevych S. V., Korpan Y. I., Sergeyeva T. A., Arkhypova V. N., Biloivan O.A., Soldatkin O. O., Shkotova L. V., Zinchenko O. A., Peshkova V. M., Saiapina O. Y., Marchenko S. V., El’skaya A. V. | |
Summary: | | |
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Edition: | Biopolymers and Cell | | | 2013,
188–206,English |
097 Thermodynamic properties of multiwall carbon nanotubes and nanocrystalline diamond |
Authors: | Vasil’ev O. О. | |
Summary: | | |
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Edition: | | | | 2013,
24 с,Ukrainian |
The events in the framework of the project |
097 097 097 097 097 097 097 097 097
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097 5. Nanobiotechnology Purpose:development and study of properties of nanostructured bioselective elements based on immobilized enzymes and carbon nanomaterials to create inexpensive, mediator-free, simple, stable, selective and highly sensitive electrochemical biosensors with improvements analytical characteristics to determine disease markers and quality control of drugs, detection of toxic compounds Expected results:Release of new product: material Stage 1:The study of physical and chemical properties of multiwalled carbon nanotubes (MWCNT) and the development of enzyme biosensors matrix based on them Stage 2:Development of bioselective membranes of enzyme sensors using carbon nanotubes and nanodiamonds and investigation of their properties Stage 3:Development of composite enzyme matrix based on carbon nanomaterials and polymers and study of their properties Stage 4:Creating of prototypes of analytical micro devices based on the nanobiocomposites developed and optimization of electrochemical measurements of toxins and metabolites in biological fluids Stage 5:Development of electrochemical biosensors prototypes based on nanostructured enzyme matrix to determine the amino acid and peptide substrates
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