Publications on the project |
096 Towards development of colorimetric test-systems for phenols detection based on computationally-designed molecularly imprinted polymer membranes |
Authors: | Т.А.Sergeyeva, L.A.Gorbach, О.А.Slinchenko, L.A.Goncharova, O.V.Piletska, О.О.Brovko, L.M.Sergeeva, G.V.Elska | |
Summary: | Colorimetric test-systems for the express control of phenols content in water were developed. The synthetic binding sites capable of phenol binding were formed in the structure of free-standing molecularly imprinted polymer (MIP) membranes. The composition of MIPs able to recognize phenol was optimized using the method of computational modeling, providing selection of functional monomers capable of interaction with phenol with high binding energy. Itaconic acid, 2-acrylamido-2-methyl-1-propanesulfonic acid, acrylamide, and methacrylic acid were identified as optimal functional monomers able to interact with phenol with high binding energies ( -34.80 kCal/M, -30.86 kCal/M, -24.14 kCal/M, and -23.17 kCal/M, respectively) and used for the synthesis of MIP membranes.
MIP membranes were obtained by co-polymerization of a functional monomer (itaconic acid / 2-acrylamido-2-methyl-1-propanesulfonic acid / acrylamide / methacrylic acid) with (tri)ethyleneglycoldimethacrylate, and oligourethaneacrylate. Addition of the elastic component oligourethaneacrylate provided the formation of a highly cross-linked polymer with receptor properties in a form of thin, flexible, and mechanically stable membrane. High accessibility of the artificial receptor sites for the interaction with analyzed phenol molecules was achieved due to the addition of a linear polymer (polyethyleneglycol Mw 20 000) to the initial monomer mixture before the polymerization. As a result typical semi-interpenetrating polymer networks (semi-IPNs) were formed. The cross-linked component of the semi-IPN was represented by the highly cross-linked MIP, while the linear one was represented by polyethyleneglycol Mw 20 000.
The phenol-selective MIP membranes were used as a basis for the colorimetric test-system for revealing phenol in aqueous samples. Phenol molecules selectively adsorbed by the MIP membranes were revealed using color reaction with 4-aminoantipyrine. The intensity of the membranes’ staining was proportional to the phenol concentration in the analyzed sample. The detection limit of phenols detection using the developed colorimetric test-system based on MIP membranes with the optimized composition is 50 nM, while the working range of the test-system - 50 nM–0.5 mM. Storage stability of test-systems at room temperature comprised 12 months. As compared to the traditional methods of phenols detection the developed test-system is characterized by simplicity of operation, compactness, and low cost.
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Keywords: | phenols, molecularly-imprinted polymers, molecularly-imprinted polymer membranes, test-systems, environmental monitoring | |
Edition: | Materials Science and Engineering C | | | 2010,
431-436,English |
096 Polymeric analytical test-systems for detection of monophenols in aqueous samples |
Authors: | L.A.Gorbach, O.O.Brovko, L.A.Goncharova, O.A.Slinchenko, L.M.Sergeeva, T.A.Sergeyeva | |
Summary: | The method of synthesis of phenol-selective molecularly imprinted polymer membranes was developed. Analytical test-systems for qualitative and semi-quantitative phenols detecton were developed on their basis. The systems function as a "litmus paper" and don't change color for several months | |
Keywords: | phenols, molecularly imprinted polymer membranes, test-systems, nanostructured polymers | |
Edition: | Polymer Journal (in Ukrainian) | | | 2010,
79–83,Ukrainian |
096 Colorimetric test-systems based on molecularly imprinted polymers for selective recognition of small organic molecules |
Authors: | L.A.Gorbach, O.O.Brovko, O.A.Slinchenko, L.A.Goncharova, L.M.Sergeeva, T.A.Sergeyeva | |
Summary: | Using the technology of molecular imprinting and a principle of synthesis of interpenetrating polymer networks molecularly imprinted (MIP) acrylaye-oligourethaneacrylate as well as composite creatinine-selective polymer membranes. Possibility of application of the MIP membranes as the novel polymeric colorimetric test-systems, working according the a "lithmus paper" principle for quantitative detection of pathological concentrations of creatinine was shown. | |
Keywords: | molecularly imprinted polymer membranes, test-systems, creatinine | |
Edition: | Ukrainian Chemical Journal | | | 2011,
59-64,Ukrainian |
096 Application of creatinine-sensitive biosensor for hemodialysis control |
Authors: | O.A.Zinchenko, S.V.Marchenko, T.A.Sergeyeva, A.L.Kukla, A.S.Pavlyuchenko, E.K.Krasyuk, A.P.Soldatkin, A.V.El’skaya | |
Summary: | The highly sensitive and selective potentiometric biosensor for creatinine determination has been developed by us earlier. In it, pH-sensitive field effect transistors were used as transducer and immobilized creatinine deiminase (EC 3.5.4.21)—as a biosensitive element. In the work presented, we optimized this biosensor for creatinine analysis in real samples of dialysate in patients with renal failure. The optimized version of biosensor was applied for on-line monitoring of the level of creatinine in the patient's dialysate fluid in the course of dialysis session. High correlation between the biosensor analysis and traditional Jaffe method was demonstrated | |
Keywords: | biosensor, creatinine, haemodyalisis control | |
Edition: | Biosensors and Bioelectronics | | | 2012,
466-469,English |
096 Colorimetric test-systems for creatinine detection based on composite molecularly imprinted polymer membranes |
Authors: | T.A.Sergeyeva, L.A.Gorbach, E.V.Piletska, S.A.Piletsky, O.O.Brovko, L.A.Honcharova, O.D.Lutsyk, L.M.Sergeeva, A.V.El’skaya | |
Summary: | An easy-to-use colorimetric test-system for the efficient detection of creatinine in aqueous samples was developed. The test-system is based on composite molecularly imprinted polymer (MIP) membranes with artificial receptor sites capable of creatinine recognition. A thin MIP layer was created on the surface of microfiltration polyvinylidene fluoride (PVDF) membranes using method of photo-initiated grafting polymerization. The MIP layer was obtained by co-polymerization of a functional monomer (e.g. 2-acrylamido-2-methyl-1-propanesulfonic acid, itaconic acid or methacrylic acid) with N, N′-methylenebisacrylamide as a cross-linker. The choice of the functional monomer was based on the results of computational modeling. The creatinine-selective composite MIP membranes were used for measuring creatinine in aqueous samples. Creatinine molecules were selectively adsorbed by the MIP membranes and quantified using color reaction with picrates. The intensity of MIP membranes staining was proportional to creatinine concentration in an analyzed sample. The colorimetric test-system based on the composite MIP membranes was characterized with 0.25 mM detection limit and 0.25–2.5 mM linear dynamic range. Storage stability of the MIP membranes was estimated as at least 1 year at room temperature. As compared to the traditional methods of creatinine detection the developed test-system is characterized by simplicity of operation, small size and low cost | |
Keywords: | molecularly imprinted polymers, molecularly imprinted polymer membranes, grafting polymerization, test-system, creatinine | |
Edition: | Analytica Chimica Acta | | | 2013,
161-168,English |
096 Rational design and development of affinity adsorbents for analytical and biopharmaceutical applications |
Authors: | E.V.Piletska, J.Kumire, T.Sergeyeva, S.Piletsky | |
Summary: | With increased level of regulations and growing consumer awareness, there is a big
demand for bioactive compounds that are sourced from sustainable, natural/biological
feedstocks. These chemicals have potential application in pharmaceutical, cosmetic
and nutraceuticals sectors. However, design and selection of selective adsorbents for
chemically diverse chemical and biological compounds from complex matrix is a very
challenging task. There is a strong call for a larger diversity of the custom-made
extraction adsorbents which would facilitate the extraction and purification of natural
and synthetic compounds for the nutraceutical and pharmaceutical industries. Equally
important is an application of affinity adsorbents and membranes in bioanalytical
applications where processing of analytical samples still remains an issue. Among the
main requirements for these adsorbents is their specificity, high binding capacity, short
time of preparation, low cost and high stability. This paper shows how computational
approach can help producing the polymeric materials possessing the required properties
quickly and effectively. The design and development strategies have been
outlined, analyzed, and illustrated by the examples of recent successes in the computational
design of the polymeric adsorbents and membranes | |
Keywords: | affinity adsorbents, molecularly imprinted polymers, membranes,
separation, purification | |
Edition: | Journal of Chinese Advanced Materials Society | | | 2013,
229-244,English |
096 Acetylcholineesterase-based conductometric biosensor for determination of aflatoxin B1 |
Authors: | О.О.Soldatkin. O.S.Burdak, T.A.Sergeyeva, V.N.Arkhypova, S.V.Dzyadevych, A.P.Soldatkin | |
Summary: | The theoretical and experimental studies were carried out, which resulted in the development ofacetylcholinesterase-based conductometric biosensor for determination of aflatoxin B1. We establisheda linear range of substrate measurement (0.25–1 mM) and optimal concentration of acetylcholine forinhibitory analysis (1 mM). The sensitivity of developed biosensor to aflatoxin B1 was tested, the cal-ibration curve was plotted for the dependence of residual activity of the acetylcholinesterase-basedbioselective element on the toxin concentration. Detection limit of aflatoxin B1 was 0.05 ug/ml. It wasshown that the developed biosensor was characterized by high signal reproducibility of direct determi-nation of the main substrate as well as of the inhibitory analysis of aflatoxin B1 (the error of detectionwas no more than 7%). | |
Keywords: | biosensor, environmental monitoring, aflatoxin B1 | |
Edition: | Sensors and Actuators | | | 2013,
999-1003,English |
096 Biosensors. A quarter of a century of R&D experience |
Authors: | A. P. Soldatkin, S. V. Dzyadevych, Y. I. Korpan, T. A. Sergeyeva, V. N. Arkhypova, O. A. Biloivan, O. O. Soldatkin, L. V. Shkotova, O. A. Zinchenko, V. M. Peshkova, O. Y. Saiapina, S. V.Marchenko, A. V. El’skaya | |
Summary: | The paper is a review of the researches of Biomolecular Electronics Laboratory concerning the development of
biosensors based on electrochemical transducers (amperometric and conductometric electrodes, potentiometric
pH-sensitive field effect transistors) and different biorecognition molecules (enzymes, cells, antibodies), biomimics
(molecularly imprinted polymers), as sensitive elements for direct analysis of substrates or inhibitory analysis
of toxicants. Highly specific, sensitive, simple, fast and cheap detection of different substances renders them as
promising tools for needs of health care, environmental control, biotechnology, agriculture and food industries.
Diverse biosensor formats for direct determination of different analytes and inhibitory enzyme analysis of a number
of toxins have been designed and developed. Improvement of their analytical characteristics may be achieved
by using differential mode of measurement, negatively or positively charged additional semipermeable membranes,
nanomaterials of different origin, genetically modified enzymes. These approaches have been aimed at increasing
the sensitivity, selectivity and stability of the biosensors and extending their dynamic ranges. During the
last 25 years more than 50 laboratory prototypes of biosensor systems based on mono- and multibiosensors for
direct determination of a variety of metabolites and inhibitory analysis of different toxic substances were created.
Some of them were tested in real samples analysis. The advantages and disadvantages of the biosensors developed
are discussed. The possibility of their practical application is considered | |
Keywords: | biosensor, immobilized enzyme, substrate, inhibitor, multibiosensor | |
Edition: | Biopolymers and Cell | | | 2013,
188-206,English |
096 Colorimetric biomimetic sensor systems based on molecularly imprinted polymer membranes for highly-selective detection of phenol in environmental samples |
Authors: | T. A. Sergeyeva, D. S. Chelyadina, L. A. Gorbach, O. O. Brovko,
E. V. Piletska, S. A. Piletsky, L. M. Sergeeva, A. V. El’skaya | |
Summary: | Aim. Development of an easy-to-use colorimetric sensor system for fast and accurate detection of phenol in environmental
samples. Methods. Technique of molecular imprinting, method of in situ polymerization of molecularly
imprinted polymer membranes. Results. The proposed sensor is based on free-standing molecularly imprinted
polymer (MIP) membranes, synthesized by in situ polymerization, and having in their structure artificial
binding sites capable of selective phenol recognition. The quantitative detection of phenol, selectively adsorbed
by the MIP membranes, is based on its reaction with 4-aminoantipyrine, which gives a pink-colored product. The
in- tensity of staining of the MIP membrane is proportional to phenol concentration in the analyzed sample.
Phenol can be detected within the range 50 nM–10 mM with limit of detection 50 nM, which corresponds to the
concentrations that have to be detected in natural and waste waters in accordance with environmental
protection standards. Stability of the MIP-membrane-based sensors was assessed during 12 months storage at
room temperature. Conclusions. The sensor system provides highly-selective and sensitive detection of phenol in
both model and real (drinking, natural, and waste) water samples. As compared to traditional methods of phenol
detection, the proposed system is characterized by simplicity of operation and can be used in non-laboratory
conditions. | |
Keywords: | phenol, molecularly imprinted polymer membranes, sensors, test-systems, colorimetry | |
Edition: | Biopolymers and Cell | | | 2014,
209-216,English |
096 096 Development of biosensor systems based on molecularly imprinted polymer membranes for detection of phenols in water |
Authors: | T.A.Sergeyeva, D.S.Chelyadina, L.A.Gorbach, O.O.Brovko, L.M.Sergeyeva, G.V.El'ska | |
Summary: | An analytical system for highly selective and sensitive detection of phenol based on molecularly imprinted polymer membranes, synthesized using method of in situ polymerization in a combination with the method of computational modeling, was developed. The detection limit for phenol detection comprised 50 nM, while the linear dynamic range – 50 nM–10 mM, which corresponds to the concentrations that are necessary to be detected in natural and waste waters. Stability of the developed MIP-based sensor systems was estimated as 12 months, which significantly increases stability of analogous devices based on receptors and enzymes. Applicability of the developed sensor systems for the analysis of phenols in both model solutions and real environmental samples (natural and waste waters) was proven. The developed systems are characterized with high selectivity, sensitivity, small sized and low cost | |
Keywords: | molecularly imprinted polymers, membranes, biosensors, test-systems, phenol | |
Edition: | Polymer Journal (in Ukrainian) | | | 2014,
289-295,Ukrainian |
The events in the framework of the project |
096 096 096 096 096 096 096 096 096 096 096
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096 5. Nanobiotechnology Purpose:development of easy-to-use, inexpensive and highly-stable optical sensor systems on the basis of nanostructured polymeric membranes synthesized using the method of molecular imprinting for detection of toxic molecules (environmental pollutants and food toxins) in the environment of food products as well as molecules-markers of some human pathologies for medical diagnostics Expected results:Issue of new types of products: methods, theories Stage 1:Synthesis of nanocomposite polymeric membranes for selective recognition of phenols and food toxins and development of sensor systems on their basis Stage 2:Development of colorimetric sensor systems for medical diagnostics Stage 3:Development of colorimetric and fluorimetric sensor systems for detection of pharmaceuticals Stage 4:Development of fluorimetric sensor systems for herbicides detection Stage 5:Testing of the developed colorimetric and fluorimetric sensor systems for environmental monitoring
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