Publications on the project |
094 Protective effect of N-stearoylethanolamine in case of violations in the tissues of the heart, liver and kidney caused by the development of carcinoma Lewis and administration of doxorubicin |
Authors: | Goudz I. A. | |
Summary: | The influence of N-stearoylethanolamine (NSE) on infringement antioxidantive protection in the heart tissue and blood plasma of rats caused by doxorubicin treatment was investigated. It was shown that doxorubicin application leads to reduction of antioxidantive protection enzymes activity in the heart tissue, superoxidedismutase, glutathioneperoxidase in particular. Aplication of the NSE promotes the partial normalization of these enzymes activity. It was shown that doxorubicin leads to urea and creatinine levels increase in blood plasma of experimental animals. NSE aplication does not influence creatinine level and normalizes the urea level. | |
Keywords: | N-stearoylethanolamine, doxorubicin, superoxidedismutase, glutathioneperoxidase, catalase, creatinine, urea. | |
Edition: | Ukrainian Biochemical Journal | | | 2014,
С. 145,Ukrainian |
094 Organ-specific effects of N-antitoxic stearoiletanolamina male mice with Lewis carcinoma in doxorubicin toxicity. |
Authors: | Goudz E.A., Gulа N.M., Goridko T.N., N. BastaY.N., Voyeikov A.I. | |
Summary: | With the introduction of doxorubicin into mice with Lewis carcinoma in heart tissues of liver and kidney were found organ-antitoxic effects of N-stearoiletanolamina (NSE), which depended on its concentration.
Administration of doxorubicin male mice leads to an increase in urea and creatinine, as well as activation of ALT in blood plasma. Introduction NSE resulted in normalization of these parameters to the level of intact animals.
In the heart tissue of doxorubicin has multi-directional effects on the activity of antioxidant enzymes, in particular decreases the activity of catalase and superoxide dismutase activity increases. Introduction NSE normalizes these two indicators.
It was found that tumor growth leads to an increase in the activity of glutathione peroxidase and superoxide dismutase. Introduction NSE normalizes activity of these enzymes. Doxorubicin causes an increase in catalase activity in the kidney of mice tumor, NSE prevented the increase in the activity of said enzyme.
During the cancer process leads to increased levels of catalase activity in the liver of tumor-bearing mice, the introduction of NSE normalized the activity of the enzyme.
| |
Keywords: | N-stearoiletanolamin, doxorubicin, heart, cardiac toxicity, nephrotoxicity, hepatotoxicity, Lewis carcinoma. | |
Edition: | Ukrainian Biochemical Journal | | | 2013,
С. 33-39,Ukrainian |
094 Antitoxic and antioxidant effects of N-stearoyetanolamin in the content of nanocomposite complex with doxorubicin in organs of mice with Lewis carcinoma. |
Authors: | Gudz E.A., Gula N.M., Horidko T.N., BashtaY.M., Berdyshev A.G., Voyeikov A.I., Kosyakova H.V.1 Panchuk R.R., Stoica R.S.
Ryabtseva A.A., Zaichenko O.S.
| |
Summary: | The aim of the study was to evaluate the possibility to reduce the doxorubicin toxic effects by its immobilization with N-stearoylethanolamine (NSE) on nanocarier polyethylene glycol. The studied parameters of the doxorubicin toxicity were: the level of creatinine in the mice blood plasma and the activity of alanine aminotransferase and aspartate aminotransferase in the blood plasma of mice. The activity of catalase superoxide dismutase, glutathione peroxidase and intensity of lipid peroxidation was determined in the tissues of the heart, kidneys and liver.
Doxorubicin in the content of nanocarrier alone caused an increase of serum creatinine and aspartateminotrasferas activity in plasma of experimental animals with carcinoma. Nanocomposite which contained doxorubicin and NSE, did not cause an increase of these parameters. It has been shown that the administration of a carrier containing doxorubicin to mice with Lewis lung carcinoma caused the decrease of the activity of catalase in mice with carcinoma. The combination of NSE and doxorubicin on the carrier led to the normalization of this parameter to the level of intact animals.
NSE immobilized on a carrier together with doxorubicin, caused a decrease in the activity of superoxide dismutase in the kidney tissue of mice with tumor. The tumor growth caused the increase of the of superoxide dismutase in mice. The administration of a carrier which contained doxorubicin and NSE normalized superoxide dismutase in heart tissue contrary of kidney. The results show the antitoxic and antioxidant effects of N-stearoylethanolamin immobilized in the nanocarrier complex together with doxorubicin.
| |
Keywords: | N-stearoylethanolamine, doxorubicin, cardiotoxicity, nephrotoxicity, hepatotoxicity, targeted drug delivery, carcinoma Lewis. | |
Edition: | Ukrainian Biochemical Journal | | | 2013,
C. 97-104,Ukrainian |
094 Antioxidantive effects of N-stearoylethanolamine in the tissue of heart, liver, buds, and in plasma of blood of mise with a carcinoma Lewis under doxorubicin treatment. |
Authors: | Goudz I., Gula N., Khmel T., Goridko T., Bashta Y., Panchuk R., Stoika R., Riabceva A., Zaichenko O. | |
Summary: | On the model of intoxication of doxorubicin in the conditions of development of carcinoma Lewis for the females of mise in fabric of heart, buds the antioxidantive effects of N-stearoylethanolamine (NSE) are rotined both a liver and plasma of blood in composition a nanocomplex and in a suspension.. The suspension of NSE reduces the level of urea in plasma blood of mise with a carcinoma Lewis, growth of which was found out as a result of introduction of doxorubicin, at introduction of nanocomplex the amount of urea remains at the level of intact mise. In plasma of blood of mise of carcinoma Lewis suspension of NSE and nanocomplex reduce activity of aspartate aminotransferase, basic marker of necrosis of fabric of heart, growth of which was caused development of tumour. Doxorubicinum is promoted by activity of alanine aminotransferase, marker of defeat of liver, in blood of mise of carcinoma Lewis, introduction of NSE in nanocomplex prevents growth of activity of enzyme. N-stearoylethanolamine modulates activity of enzymes of antioxidantive protection of tissue of heart, kidney and liver of mise with a carcinoma Lewis. | |
Keywords: | N-stearoylethanolamine, doxorubicin, heart, kidney, liver, delivery of medications systems | |
Edition: | Ukrainian Biochemical Journal | | | 2012,
С. 61-69.,Ukrainian |
094 Antioxidative effect of the N-stearoylethanolamine in the heart tissue and blood plasma of rats under doxorubicin treatment. |
Authors: | Goudz I,. Gula N,. Khmel T,. Goridko T,. Berdushev A. | |
Summary: | The influence of N-stearoylethanolamine (NSE) on infringement antioxidantive protection in the heart tissue and blood plasma of rats caused by doxorubicin treatment was investigated. It was shown that doxorubicin application leads to reduction of antioxidantive protection enzymes activity in the heart tissue, superoxidedismutase, glutathioneperoxidase in particular. Aplication of the NSE promotes the partial normalization of these enzymes activity. It was shown that doxorubicin leads to urea and creatinine levels increase in blood plasma of experimental animals. NSE aplication does not influence creatinine level and normalizes the urea level. | |
Keywords: | N-stearoylethanolamine, doxorubicin, superoxidedismutase, glutathioneperoxidase, catalase, creatinine, urea. | |
Edition: | Ukrainian Biochemical Journal | | | 2011,
С. 86-91.,Ukrainian |
The events in the framework of the project |
|
094 5. Nanobiotechnology Purpose:Justification efficiency combined effect of anti-tumor factors (for example, doxorubicin) in the new nanoscale carriers functionalized derivatives endokannabinoyids (N-acylethanolamines) on malignant cells of various origins in vitro and in vivo Expected results: Stage 1:Investigation of the effect of the combined action of doxorubicin and N-acylethanolamines the cells and tissues of the mouse and human Stage 2:Research actions nanocomposite of doxorubicin and N-acylethanolamines the cells and tissues of the mouse and human Stage 3:Study of apoptosis in cells of mouse and human action nanocomposite of doxorubicin and N-acylethanolamines in vitro and in vivo Stage 4:Study of cytoprotective activity in vitro and in vivo nanocomposite of N-acylethanolamines and doxorubicin Stage 5:The study therapeutic efficacy of doxorubicin nanocomposite and N-acylethanolamines in vivo in models of experimental tumors of mice
|