09077 09091 09092 09093 09094
Publications on the project |
090 Method for quantitative evaluation of reactive oxygen species changes in cells in vivo under impact of metal-containing nanomaterials |
Authors: | Chekhun V.F., Naleskina L.A., Lukianova N.Yu., Shvets Yu.V., Todor I.M., Demash D.V., Lozovska Yu.V. | |
Summary: | Method for quantitative determination of changes of active forms of oxygen in cells in the in vivo system under effect of nano-particles of metal-containing materials. The determination of the changes of generation of active forms of oxygen under effect of nano-particles of magnetic fluid and nano-particles of colloid gold is carried out directly in tumor cells stained with specific dye, without solution of continuity of those by means of flow cytofluorometry. | |
Keywords: | Reactive oxygen species, flow cytofluorimetry, magnetic fluid, colloid gold, malignant cells | |
Edition: | | | | 2014,
,Ukrainian |
090 Cytotoxic effects of metal-containing nanomaterials in tumor cells in vivo |
Authors: | V.F. Chekhun, L.A. Naleskina, I.N. Todor, Yu.V. Shvets, Yu.V. Lozovskaya, D.V. Demash, N.Yu. Lukianova | |
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Edition: | | | | 2014,
493-497,Russian |
090 In vivo genotoxicity of ferromagnetic nanoparticles and static magnetic field |
Authors: | Lozovska Yu.V., Todor I.M., Demash D.V., Lukianova N.Yu., Khayetsky I.K., Naleskina L.A., Chekhun V.F. | |
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Edition: | | | | 2011,
218-219,Ukrainian |
090 A method for verification of cyto-toxic effects of metal-containing nano-materials on tumor cells |
Authors: | Chekhun V.F., Nalieskina L.A., Lukianova N.Yu., Todor I.M., Kunska L.M., Demash D.V. | |
Summary: | A method for verification of cyto-toxic effects of metal-containing nano-materials on tumor cells wherein for the determination of the level of cyto-toxic effect of metal-containing nano-agent on cyto-architectonics of cells depending on its concentration and duration of contact action one uses complexes of simple, not labor-consuming cyto-morphological characteristics of changes in cyto-plasma of tumor cells – change of configuration of cytoplasm, effect of blebbing of cytoplasm membrane, vacuolar hydropic degeneration, and in nuclei – premature condensation of chromosomes, re-distribution of chromatin, fragmentation of nuclei, formation of protrusions and micro-nuclei. | |
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Edition: | | | | 2012,
,Ukrainian |
090 A method for evaluation of biological safety of nano-materials in vitro |
Authors: | Chekhun V.F., Dolynskyi H.A., Nalieskina L.A., Lukianova N.Yu., Todor I.M., Demash D.V. | |
Summary: | A method for evaluation of biological safety of nano-materials in vitro that is characterized in that the measurement of intensity of luminol-dependent chemoluminescence is performed in the presence of diperiodat cuprate in culture of cells that are affected by the tested nano-material, and if it exceeds the background level of luminescence the conclusion is made on biological danger of the nano-material under investigation. | |
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Edition: | | | | 2013,
,Ukrainian |
The events in the framework of the project |
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090 5. Nanobiotechnology Purpose:To study dynamics of free radicals accumulation in malignant cells in vitro as a result of cytotoxic action of nanosized materials of different origin. Expected results:Issue of new types of products: methods, theories Stage 1:Study of features of accumulation and distribution of nanomaterials including ferromagnetics in cells in vitro and development of a method of light-optical ferromagnetic vizualisation in tumor cells. Stage 2:Determination of impact caused by nanosized materials of different origin on cytomorphological features, cell cycle and cell ultrastuctural organisation in vitro. Stage 3:Study of free radicals accumulation dynamics in cell systems in vitro as a result of cytotoxic activity of nanosized materials of different origin. Stage 4:Determaintion of cytotoxic impact of nanomaterials of different origin on cellular lipid and energetic metabolism in vitro. Stage 5:Evaluation of endogenous and exogenous iron ratio in tumor cells under impact of nanomaterials of different origin. Development of algorithm for testing of new nanovector systems on tumor cells.
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