5. Nanobiotechnology
Purpose:Development of methods for efficient delivery of therapeutic agents to tumour cells for block their growth
Expected results:Issue of new types of products: methods, theories
Stage 1:Selection and physic-chemical studies of optimal nonporous silica matrix and branching and a linear polymer with dextran core for the target delivery of drugs at leukaemia cells. Design and synthesis of specific oligonucleotides against oncogenes mRNA. Modification matrix specific to M3-M5 leukaemia, siRNA, oligonucleotides and radioisotopes with different half-lives. Testing the efficiency of the matrix in vitro.
Stage 2:Obtain primary temporary lines from patients with chronic myelomonocytic leukemia (HMML) monoblastic acute leukemia (M5 HML) myelomonoblastic acute leukemia, solid tumors, infiltrated neutrophils, malignant histiocytic disease. Selection and physico-chemical studies of optimal porous silica matrix for target delivery of drugs in leukemia cells. Modification silica matrix and branching linear polymer with core-specific dextran M3 M5 leukemia drugs, siRNA, oligonucleotides and isotopes with different types of radiation. Testing the efficiency of the system matrix in vitro
Stage 3:Selection and physico-chemical studies of optimal nonporous silica matrix for target delivery of drugs in leukemia cells. Physico-chemical characterization matrices. Modification silica matrix and nanoparticles on the basis of branching and linear polymer core dextran specific to leukemia M3-M5 antisens oligonucleotides, different isotopes. Testing the efficiency of the system matrix in vitro
Stage 4:Selection and physico-chemical studies of optimal porous silica matrix for target delivery of drugs in leukemia cells. Modification silica matrix and nanoparticles on the basis of branching and linear polymer core dextran specific to leukemia M3-M5 antisens oligonucleotides and different isotopes. Determining the structure of the surface adsorption layer interaction energy parameters determining the adsorbed molecules with surface active centers. Testing the efficiency of the system matrix in vitro
Stage 5:The final selection of the optimum modified nanoconstructions and check them for temporary leukocyte cultures M3-M5 leukemia and model animals