Publications on the project |
082 USE OF THYMINE AUXOTROPHIC MUTANTS OF ERWINIA CAROTOVORA |
Authors: | Romaniuk L.V., Mukvych M.S., Ivanytsya T.V., Tovkach F.I. | |
Summary: | It is shown for the first time that pectolytic erwinia exist in nature not only as defect-polylysogenic system, but also as those which carry prophages of viable bacteriophages. Multiple lysogeny in Erwinia carotovora subsp. carotovora ZM1, is characterized by defective and viable phages, colicin-like bacteriocins which may represent the products of phage synthesis. Use of thymine auxotrophic mutants for obtaining defective bacteriophage particles allowed to create a sensitive system, which can be used to obtain selectively different types of bacteriocins with high killer activity. In the future, the process of scaling a less cost- and time-consuming method of induction by thymine starvation auxotrophs, can create conditions for propagation of
bacteriocins in large quantities and opens the prospect for obtaining individual phage components that can be
used in nanotechnology as a variety of matrixes. | |
Keywords: | Erwinia carotovora, bacteriophage, defective phage particles, carotovoricins, killer activity. | |
Edition: | | | | 2011,
,Russian |
082 Особенности морфогенетического развития вирулентных мутантов єрвиниофага ZF40 |
Authors: | Korol N.A., Romaniuk L.V., Ostapchuk A.M., Ivanytsya T.V., Kushkina A.I, Tovkach F.I. | |
Summary: | | |
Keywords: | | |
Edition: | | | | 2011,
,Russian |
082 CHARACTERISTICS OF DEFECTIVE PHAGE PARTICLES OF PECTOBACTERIUM CAROTOVORUM ZM1 |
Authors: | Tovkach F.I., Ivanytsia T. V., Kushkina A.I. | |
Summary: | It is shown for the first time that the expression products of defective prophages are typical of defective lysogenic systems of phytopathogenic Pectobacterium carotovorum. It is established that virus-like particles (VLP) such as phage capsids are packing bacterial DNA which size is determined by pulse field gel electrophoresis separation. Based on data about capsid structures which are formed by the virulent mutant ZF40/421, there is made a suggestion about the forming mechanism of defective virions of P. carotovorum. | |
Keywords: | Pectobacterium carotovorum, defective lysogeny, phage, virus-like particles (VLP) | |
Edition: | | | | 2012,
,English |
082 Long-term preservation of unstable bacteriophages of Enterobacteria |
Authors: | Tovkach F.I., Zhuminska G.I., Kushkina A.I. | |
Summary: | Bacteriophages are integral components of bacterial communities. Their practical applications and significance for humans are various. Thus, keeping phage collections along with their specifi c host bacteria is an urgent and important mission for biologists. The problems of the long-term storage of phages steel are not completely solved. The main diffi culties may occur due to the structural instability of virions as well as an accelerated genetic variability in phage genomes both in vivo and in vitro. In the paper the results of 10-years observation over unstable bacteriophage storage process was presented as well the method of their long-term preservation was proposed. It consisted of the optimization of STMG buffer system (200 mM NaCl, 10 mM Tris-HCl, pH 7.4, 1 mM MgCl2, 100 μg/ml gelatin) [Serwer P., Pichler M.E. Electrophoresis of bacteroiphage T7 capsids in agarose gels// J.Virol. – 1978. – V.28, N3. – P.917-928] by higher gelatin concentration or its replacement by fi coll (2 – 6 %), and increasing of Mg2+ concentration to 10 mM. The proposed buffer composition allowed saving structural entirety of unstable phage virions during their long-term storage. | |
Keywords: | Bacteriophages, long-term storage, structural instability, gelatine,fi coll. | |
Edition: | | | | 2012,
,English |
082 New Approach for Identification of Bacteriophage Virion Structural Proteins |
Authors: | Korol N.A., Tovkach F.I. | |
Summary: | The ability of the phage structural polypeptides to undergo post-translational modification makes the task of correlation of the primary nucleotide sequence data with the actual structural proteins of a virion extremely challenging. This study describes an alternative model approach based on two-stage chromatography for allocation of virion structural components and identification of their major polypeptides. Bacteriophage T4D, its amber mutant T4D23 (amH11) and its tail preparations were purified, concentrated and separated by ion exchange chromatograpgy based on fibrous DEAE-cellulose. The major tail fraction was then exposed to size-exclusion chromatography which enabled to separate tail components by size. This method proved itself as a highly efficient and gentle enough to save most of the biological material without changing the basic properties of the native phage. The result also shows that the accumulation of individual phage tails in the course of the amber mutant T4D23 (amH11) propagation on the permissive host Escherichia coli CR63 was resulted by changes in the conditions of reproduction. The ability of bacteriophages to form an excess of tails, capsids and other structures during reproduction on a non-traditional host provides an alternative way for obtaining highly concentrated preparations of virion components for further analysis of their major proteins and determination of the genes responsible for their synthesis. | |
Keywords: | Bacteriophage T4, amber mutant, virion components, ion-exchange, size-exclusion chromatography, polypeptides.
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Edition: | | | | 2013,
,English |
The events in the framework of the project |
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082 5. Nanobiotechnology Purpose: Expected results:Release of new product: material Stage 1: Stage 2: Stage 3: Stage 4: Stage 5:
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