Publications on the project |
073 Silver and gold nanoparticles on sol-gel TiO2, ZrO2, SiO2 surfaces: optical spectra, photocatalytic activity, bactericide properties |
Authors: | Eremenko A.M., Smirnova N.P., Gnatyuk Yu. .I, Linnik O.P., Mukha Yu.P., Korduban A.M. | |
Summary: | | |
Keywords: | | |
Edition: | Composite materials | | | 2011,
51-82,English |
073 Influence of Gold Nanoparticles on the Aerosil Surface on the Fluorescence and Raman Scattering Spectra of Adsorbed Tryptophan |
Authors: | A. M. Eremenko, N. P. Smirnova, I. P. Muha, A. P. Naumenko, N. M. Belyy, S. Hayden
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Summary: | The fluorescence (Fl) and Raman scattering spectra of the aminoacid tryptophan (Trp) in the presence of
gold nanoparticles (Au NPs) in solution and on the surface of highly dispersed silica (Au-SiO2) are dis-
cussed. Fl of Trp is efficiently quenched in the presence of Au Nps both in solution and on the surface. The
Fl and excitation spectra demonstrate the bands of molecular Trp and charge-transfer complex between
Trp and NPs. There is a significant increase in the intensity of the carboxyl and amino groups as well as
the benzene and pyrrole ring vibrations in the Raman spectra of Trp in presence of Au NPs. Influence of
gold nanoparticles on the Raman spectra of Tpn in a heterogeneous system is severely weakened due to
the strong scattering of light by dispersed silica.
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Keywords: | gold nanoparticles, tryptophan, silica, fluorescence, SERS | |
Edition: | International Journal of Surface Science | | | 2012,
49-55, |
073 Antimicrobial Activity of Stable Silver Nanoparticles of a Certain Size |
Authors: | Iu. P. Mukha, A. M. Eremenko, N. P. Smirnova, A. I. Mikhienkova, G. I. Korchak, V. F. Gorchev, and A. Yu. Chunikhin | |
Summary: | Conditions for obtaining stable silver nanoparticles smaller than 10 nm were developed using a
binary stabilizer polyvinylpyrrolidone/sodium dodecylsulphate in optimal ratio. Optical spectra, morphology
and dependence of size of the nanoparticles on the amount of reducing agent were studied. Colloidal soluu
tions of nanosilver showed a high bactericidal activity against strains of Staphylococcus aureus, Escherichia coli
and Pseudomonas aeruginosa, and fungicidal activity against Candida albicans. The mechanism of action of
nanosized silver on microbial cell was examined by laser scanning confocal microscope using fluorescent
label. First step of antimicrobial effect on microorganisms was membrane damage and penetration of silver
nanoparticles into the cell. Prolonged stability of nanoparticles and their antimicrobial activity over the past
two years were showed. | |
Keywords: | | |
Edition: | Applied Biochemistry and Microbiology | | | 2013,
215–223,Russian |
073 Comparative spectral behavior of tryptophan in Au/SiO2 and Cu/SiO2 nanocomposites |
Authors: | A.M. Eremenko, I.S. Petrik, Iu.P. Mukha, N.P. Smirnova, S. Hayden, A.P. Naumenko | |
Summary: | The peculiarities of fluorescence and Raman spectra of amino acid tryptophan (Trp) on the surface of dispersed
silica containing gold (Au/SiO2) and copper (Cu/SiO2) nanoparticles (NPs) have been studied. IR- and UV-spectra
of Cu-containing systems revealed that Cu NPs coexist with copper oxides in solution and on the surface of SiO2.
The fluorescence of Trp is efficiently quenched in the presence of gold and copper NPs both in solution and on the
SiO2 surface due to charge transfer processes between Trp and metal NPs. The intensities of the vibration bands of
benzene and pyrrole rings as well as carboxyl and amino groups of Trp in Raman spectra in the presence of Au NPs
are greatly enhanced. Whereas in the presence of Cu NPs the enhanced vibration of Trp occurred for carboxyl
groups and benzene ring adsorbed on Cu-OH groups resulted from Cu NPs oxidation. | |
Keywords: | gold, copper, nanoparticles, tryptophan, enhanced Raman spectra, fluorescence quenching | |
Edition: | International Journal of Surface Science | | | 2012,
1-9,English |
073 Antibacterial Action and Physicochemical Properties of Stabilized Silver and Gold Nanostructures on the Surface of Disperse Silica |
Authors: | I. Mukha, А. Eremenko, G. Korchak, А. Michienkova | |
Summary: | This work is devoted to the synthesis and stabilization of nanosized Ag/SiO2 and Au/SiO2 disperse materials and investigation their morphology, optical and antimicrobial properties. First, Ag and Au nanoparticles (NPs) were produced in colloids via chemical (Ag) or photochemical (Au) reduction of appropriate ions. To prevent the oxidation of Ag NPs in colloid solution, external binary stabilizing agents PVP and SDS were used. Then, Ag and Au NPs (0.01-0.05% wt) were adsorbed from their colloid solutions on high disperse silica surface (Ssp=260m2/g) and samples prepared were dried. Materials obtained were studied by UV-vis, XRD, and TEM methods. Ag and Au NPs adsorbed on silica demonstrated a fair crystallinity in XRD. The surface plasmon resonance (SPR) band positions inherent to Ag and Au NPs on silica surface as well as the intensities of optical spectra were stable during 7 month and more. Obtained Ag NPs in colloids and Ag/SiO2 composites demonstrated excellent antimicrobial activity against a series of the microorganisms (Escherichia coli, Staphylococcus aurous, and Candida albicans). Au/SiO2 samples did not reveal any bactericide properties relative to the test microorganisms grown. The mechanisms of Ag(Au) NPs interaction with silica surface were analyzed. | |
Keywords: | Silver, Gold Nanoparticles, Binary Stabilizer PVP and SDS, Adsorption, High Disperse Silica, Antimicrobial Activity | |
Edition: | J. Water Resource and Protection, | | | 2010,
131-136,English |
073 Silver and Gold Nanoparticles in Silica Matrices: Synthesis, Properties, and Application |
Authors: | À. M. Eremenko, N. P. Smirnova, I. P. Mukha, H. R. Yashan | |
Summary: | Synthesis, optical spectra, morphology, catalytic and bactericide properties of the silica-based composite
films and powders, modified with silver, gold and bimetallic Ag/Au alloy and core-shell nanoparticles are
considered. Photochemical reduction of Au3+ and Ag+ ions with silica-adsorbed benzophenone molecules
promoted the formation of stable Ag and Au nanoparticles in solution for the further introduction into the
silica. The application of nanosized composites in catalytic experiments and as biocides are discussed. | |
Keywords: | nanoparticles, gold, silver, photoreduction, optical spectra, nanoparticle structure, sol-gel process, bactericidal
properties | |
Edition: | | | | 2010,
67-86,Russian |
073 Cyto- fnd genotoxic influence of of colloidal Ag and Au nanopreparations on primary cell cultures |
Authors: | І.О.Shmarakov, M M.Marchenko, Yu.P.Mukha, G.R.Yashan, N.P.Smirnova, G.M.Eremenko | |
Summary: | The estimation of cyto- and getotoxicity of mono- and bimetallic nanoparticles on primary cell cultures of lympho-cytes, hepatocytes and Guerin’s carcinoma cells was performed. It is determined that the primary mechanism of nano-particles toxicity is disruption of cell membrane structural integrity. At further incubation (48 hours) the features of oxidative stress are observed, which is expressed in the increase of TBA-active products, protein carbonyl derivatives, nuclear DNA disruption (majority of С3-С4 comets, Idc=3,2) and metabolic activity decrease. | |
Keywords: | nanoparticles Аg and Аu, cytotoxicity,oxidative protein modification, TBA-active products, genotoxicity | |
Edition: | | | | 2010,
13-20,Ukrainian |
073 Characteristic and stability of antimicrobial effect of silver nanoparticles in colloid solutions |
Authors: | Mikhienkova A., Mukha Yu. | |
Summary: | It was established in the experiment that colloid
solution of silver nanoparticles, obtained by
chemical method with the particle size of 8412 nm
and concentration of basic solution 0,0016%
showed an antimicrobial activity for test4microor4
ganisms E. coli, P. aeruginosa, S. aureus and
C. albicans. It depended upon a number of
factors. A mixture of surface4active sodium dode4
cyl sulphate and polymer polyvinylpyrrolidone was
used as a stabilizing substance. Impact of the
nature of stabilizing substances on the preservation of antimicrobial activity of experimental
solutions was studied and their stability during a
long period of time was also demonstrated. | |
Keywords: | | |
Edition: | Environment & health | | | 2011,
55-59,Russian |
073 Synthesis and stabilization of Cu nanoparticles in aqueous solutions and their bactericidal activity |
Authors: | Petrik I.S., EremenkoA.M., Smirnova N.P., Korchak G.I., Mikhiyenkova A.I. | |
Summary: | Colloids of copper nanoparticles were synthesized by reduction of copper sulfate and copper acetate
in aqueous solutions using sodium tetrahydroborate in the presence of potassium iodide at room
temperature. These particles are unstable in comparison with those of silver and gold, especially in the
air. Therefore, the additional amount of stabilizers: polyethylene glycol, sodium dodecyl sulfate, colloidal
silica and nonionic amphiphilic triblocks copolymer Pluronic have been used. It has been found that
stability of Cu increases after stabilizer adding to the fresh-prepared nanoparticles covered with iodine
ions. It has been shown that copper nanoparticles have a high bactericidal activity against bacteria
Escherichia coli, higher than that of Cu2+ ions as well as oxide layer formed on the Cu nanoparticle
surface. Bactericidal activity of CuO is comparable with that of related ions. | |
Keywords: | copper nanoparticles, bactericidal activity, stabilizer, aqueous solution, Escherichia coli | |
Edition: | Chemistry, physics and technology of surface | | | 2014,
74-81,Ukrainian |
073 Antimicrobial activity of stabilized silver and copper nanoparticles in colloids and on the silica surface |
Authors: | Anna Eremenko, Irina Petrik, Iuliia Mukha, Natalia Smirnova, Anna Mikhienkova, Galina Korchak | |
Summary: | Silver and copper nanoparticles (NPs) with high antimicrobial properties are synthesized in colloids and on the dispersed silica surface. Optical spectra, morphology and antimicrobial activity of nanoparticles are investigated.
Ag NPs demonstrate high bactericidal activity against strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and fungicidal activity against Candida albicans. Cu NPs are effective as bactericides against Escherichia coli. The CuO oxide layer was formed on the surface of Cu NPs with a further decrease of the metallic core size. Bactericidal activity of suspension Cu/SiO2 is high and comparable to that of Ag NPs.
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Keywords: | silver, copper nanoparticles, silica, SPR spectra, morphology, bactericides | |
Edition: | PROC. CERECO | | | 2014,
146-151,English |
073 Tryptophan-Assisted Synthesis Reduces Bimetallic Gold/Silver Nanoparticle Cytotoxicity and Improves Biological Activity |
Authors: | Igor O. Shmarakov, Iuliia P. Mukha, Volodymyr V. Karavan, Olexander Yu. Chunikhin, Mykhailo M. Marchenko, Natalia P. Smirnova and Anna M. Eremenko | |
Summary: | Aiming to reduce the potential in vivo hepato-and nephrotoxicity
of Ag/Au bimetallic nanoparticles (NPs) stabilized
by sodium dodecyl sulphate (SDS), an approach
involving a simultaneous reduction of silver nitrate and
tetrachlorauratic acid using tryptophan (Trp) as a reducing/
stabilizing agent was applied during NP synthesis. The
obtained Ag/Au/Trp NPs (5-15 nm sized) were able to form
stable aggregates with an average size of 370-450 nm and
were potentially less toxic than Ag/Au/SDS in relation to a
mouse model system based on clinical biochemical parameters
and oxidative damage product estimation.
Ag/Au/Trp NPs were shown to exhibit anticancer activity
in relation to a Lewis lung carcinoma model. The data
generated from the present study support the fact that the
use of tryptophan in NP synthesis is effective in attenuating
the potential hepatotoxicity and nephrotoxicity of NPs
during their in vivo application. | |
Keywords: | gold, silver, nanoparticles, tryptophan-assisted
synthesis, hepatotoxicity, anticancer activity | |
Edition: | Nanobiomedicine | | | 2014,
1-10,English |
The events in the framework of the project |
073 073 073 073 073 073 073 073 073 073
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073 5. Nanobiotechnology Purpose: Expected results:Release of new product: technology Stage 1: Stage 2: Stage 3: Stage 4: Stage 5:
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