Founded in 1964.
Main areas of research activities:
- studies in the field of industrial botany;
- introduction and acclimatization of plants, aimed at enriching plant resources, using them in the national economy and optimizing industrially loaded environments;
- development of scientific fundamentals for vegetation preservation in the industrial regions of South-East Ukraine.
Principal achievements and developments:
- A unique collection stock of living plants (over 9 thousand species, varieties, forms and cultivars) of the international level has been created in conditions of arid steppe and industrial pollution, including a collection and displays of tropical and subtropical plants, which enjoy the National Asset status.
- Over 50 plant varieties resistant to hard steppe conditions have been produced.
- A methodology and respective technologies have been developed for phytooptimization of industrial environment and amelioration of industrially polluted and agricultural lands.
- Physiological, biochemical, populational-genetics mechanisms of plant tolerance to human impacts have been studied.
- The composition and structure of the natural flora and vegetation of Ukrainian South-East have been determined, processes of their anthropogenic transformation studied, scientific principles of plant-diversity preservation in industrial regions and formation of a regional ecological network developed.
- A technique for controllable enforced complete restoration of steppe phytocenoses has been worked out; simulative models of steppes that are the national asset of Ukraine have been developed on its basis.
Research results of Donetsk Botanical Garden have been implemented in the following applied developments:
- a technology for phyto-reclamation of the lands degraded by industry;
- a technology for amelioration of forage areas;
- a technology for enforced restoration of steppe vegetation;
- substantiation study for setting up a regional ecological network;
- population-genetics method for forming conifer plantations;
- plant indication and monitoring of industrial air and biological pollution;
- a technology for the accelerated propagation of plants.
Artifical steppe as a part of complex exposition " Steppes of Ukraine" which is the national property of Ukraine of the Donetsk Botanical Garden of the National Academy of Science of Ukraine 
Arid region plants exposiotion as a part of object which is the national property of Ukraine of the Donetsk Botanical Garden of the National Academy of Science of Ukraine 
Selection breeding plot of the chrysanthemum of the Donetsk Botanical Garden of the National Academy of Science of Ukraine 
At arboretum of the Donetsk Botanical Garden of the National Academy of Science of Ukraine